Recombinant Human Tryptase Alpha/Beta 1, His-tagged

• Cat.No.: TPSAB1-7161H
• Product Overview: Recombinant human TPSAB1 protein, fused to His-tag at N-terminus, was expressed in E. coli. MW: 30.1 kDa.

Specification

• Description: TPSAB1, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea.
• Molecular Mass: 30.1 kDa(270aa)
• Protein length: 31-275aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1.0 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TPSAB1 tryptase alpha/beta 1 [ Homo sapiens ]
• Official Symbol: TPSAB1
• Synonyms: TPSAB1; tryptase alpha/beta 1; TPS1, TPS2, TPSB1, tryptase beta 1; tryptase alpha/beta-1; tryptase alpha II; tryptase beta I; tryptase I; tryptase II; tryptase III; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta-1; tryptase alpha-1; mast cell beta I tryptase; mast cell alpha II tryptase; TPS1; TPS2; TPSB1; MGC104064
• Gene ID: 7177
• mRNA Refseq: NM_003294
• Protein Refseq: NP_003285
• MIM: 191080
• UniProt ID: Q15661
• Chromosome Location: 16p13.3
• Pathway: Activation of Matrix Metalloproteinases, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem
• Function: peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity