"POLR2J3" Related Products

Recombinant Human POLR2J3 polymerase (RNA) II (DNA directed) polypeptide J3, His-tagged

Cat.No.: POLR2J3-7175H
Product Overview: Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 15.5 kDa.
Description: DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% glycerol,1mM DTT.
Molecular Mass: 15.5 kDa (138aa) confirmed by MALDI-TOF
Protein length: 1-115aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
Purity: >95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: POLR2J3 polymerase (RNA) II (DNA directed) polypeptide J3 [ Homo sapiens ]
Official Symbol: POLR2J3
Synonyms: POLR2J3; polymerase (RNA) II (DNA directed) polypeptide J3; POLR2J2; RPB11b1; RPB11b2; DNA-directed RNA polymerase II subunit RPB11-b2; RNA polymerase II subunit B11-b2; DNA-directed RNA polymerase II subunit 11; DNA-directed RNA polymerase II subunit J3
Gene ID: 548644
mRNA Refseq: NM_001097615
Protein Refseq: NP_001091084
UniProt ID: Q9GZM3
Chromosome Location: 7q22.1
Pathway: Epstein-Barr virus infection, organism-specific biosystem; Huntington"s disease, organism-specific biosystem; Purine metabolism, organism-specific biosystem; Pyrimidine metabolism, organism-specific biosystem; RNA polymerase, organism-specific biosystem
Function: DNA binding; DNA-directed RNA polymerase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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