Active Recombinant Human ABL1, GST-tagged

• Cat.No.: CABLES1-1361H
• Product Overview: Recombinant human ABL1 (G250E) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: ABL1protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 6 nmol/min/mg
• Molecular Mass: ~160 kDa
• Protein length: 27 aa-end
• Purity: >70%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: CABLES1 Cdk5 and Abl enzyme substrate 1 [ Homo sapiens ]
• Official Symbol: CABLES1
• Synonyms: CABLES1; Cdk5 and Abl enzyme substrate 1; CDK5 and ABL1 enzyme substrate 1; FLJ35924; HsT2563; interactor with CDK3 1; CABL1; IK3-1; CABLES
• Gene ID: 91768
• mRNA Refseq: NM_001100619
• Protein Refseq: NP_001094089
• MIM: 609194
• UniProt ID: Q8TDN4
• Chromosome Location: 18q11.2
• Pathway: Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: cyclin-dependent protein kinase regulator activity