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Active Recombinant Human MYLK2, His-tagged

Cat.No. : MYLK2-1435H
Product Overview : Recombinant full-length human MYLK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
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Description : MYLK2 is a member of the myosin light chain kinase family and is a calcium/calmodulin dependent enzyme that is exclusively expressed in adult skeletal muscle. MYLK2 has been proposed to participate in signaling pathways (calcium signaling pathway, focal adhesion, regulation of actin cytoskeleton) and cellular processes (neuromuscular synaptic transmission, protein/amino acid phosphorylation). MYLK2 is involved in multiple molecular functions as a result of various subdomains that participate in ATP binding, calmodulin binding, nucleotide binding, protein serine/threonine kinase activity and transferase activity).
Source : Sf9 Cells
Species : Human
Tag : His
Form : Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.2mM DTT, 25% glycerol.
Bio-activity : 270 nmol/min/mg
Molecular Mass : ~74kDa
Protein length : Full length
Purity : >75%
Applications : Kinase Assay, Western Blot
Storage : Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
Concentration : 0.1μg/μl
Gene Name : MYLK2 myosin light chain kinase 2 [ Homo sapiens ]
Official Symbol : MYLK2
Synonyms : MYLK2; myosin light chain kinase 2; myosin light chain kinase 2, skeletal muscle; myosin light chain kinase 2, skeletal/cardiac muscle; KMLC; MLCK2; skeletal muscle myosin light chain kinase; skMLCK; MLCK;
Gene ID : 85366
mRNA Refseq : NM_033118
Protein Refseq : NP_149109
MIM : 606566
UniProt ID : Q9H1R3
Chromosome Location : 20q13.31
Pathway : Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Focal Adhesion, organism-specific biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Gastric acid secretion, organism-specific biosystem; Gastric acid secretion, conserved biosystem;
Function : ATP binding; calmodulin binding; calmodulin binding; calmodulin-dependent protein kinase activity; myosin light chain binding; myosin light chain kinase activity; myosin light chain kinase activity; nucleotide binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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