Active Recombinant Human PIK3C3, GST-tagged

• Cat.No.: PIK3C3-1454H
• Product Overview: Recombinant full-length human PIK3C3 (Vps34) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PIK3C3 or phosphoinositide-3-kinase is a member of the PI 3-kinase family that mediates the active diversion of proteins from the secretory pathway to vacuoles. PIK3C3 are involved in both receptor-mediated signal transduction and intracellular trafficking. In yeast, the vps34 gene product is a PI 3-kinase. PIK3C3 has a substrate specificity and ion requirements that are distinct from the other known mammalian PI 3-kinases. PIK3C3 mediate catalysis on membranes and suppress futile adenosine triphosphatase cycles. PIK3C3 appears to alternate between a closed cytosolic form and an open form on the membrane.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 98 nmol/min/mg
• Molecular Mass: ~128 kDa
• Protein length: Full length
• Purity: >70%
• Applications: Kinase Assay, Western Blot
• Storage: Store at–70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PIK3C3 phosphoinositide-3-kinase, class 3 [ Homo sapiens ]
• Official Symbol: PIK3C3
• Synonyms: PIK3C3; phosphoinositide-3-kinase, class 3; phosphatidylinositol 3-kinase catalytic subunit type 3; Vps34; PI3K type 3; PI3-kinase type 3; PtdIns-3-kinase type 3; phosphatidylinositol 3-kinase p100 subunit; VPS34; hVps34; MGC61518
• Gene ID: 5289
• mRNA Refseq: NM_002647
• Protein Refseq: NP_002638
• MIM: 602609
• UniProt ID: Q8NEB9
• Chromosome Location: 18q12.3
• Pathway: 3-phosphoinositide biosynthesis, organism-specific biosystem; 3-phosphoinositide biosynthesis, conserved biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Inositol phosphate metabolism, organism-specific biosystem
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; nucleotide binding; protein binding; protein kinase activity