"SPHK1" Related Products

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Recombinant Human SPHK1 (Active), GST-tagged

Cat.No.: SPHK1-1483H
Product Overview: Recombinant full-length human SPHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description: SPHK1 catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP) which is a novel lipid messenger with both intracellular and extracellular functions. Various stimuli increase cellular levels of SPP by activation of SPHK1. Competitive inhibitors of SPHK1 block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors. SPHK1/sphingosine-1-phosphate pathway contributes to colon carcinogenesis. SPHK1 plays a key role in the generation and release of proinflammatory mediators triggered by anaphylatoxins from human macrophages and also is involved in the process of immune cell motility.
Source: Sf9 Cells
Species: Human
Tag: GST
Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Bio-activity: 450 nmol/min/mg
Molecular Mass: ~71 kDa
Protein length: Full length
Purity: >70%
Applications: Kinase Assay, Western Blot
Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
Concentration: 0.1μg/μl
Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
Official Symbol: SPHK1
Synonyms: SPHK1; sphingosine kinase 1; SPHK; SK 1; SPK 1;
Gene ID: 8877
mRNA Refseq: NM_001142601
Protein Refseq: NP_001136073
MIM: 603730
UniProt ID: Q9NYA1
Chromosome Location: 17q25.2
Pathway: Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chaperonin-mediated protein folding, organism-specific biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Fc-epsilon receptor I signaling in mast cells, organism-specific biosystem;
Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; magnesium ion binding; nucleotide binding; protein binding; protein phosphatase 2A binding; sphinganine kinase activity; transferase activity;

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