Active Recombinant Human PTPN1, GST-tagged

• Cat.No.: PTPN1-1517H
• Product Overview: Recombinant full length human PTPN1 was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Specification

• Description: Protein tyrosine phosphatase nonreceptor, type 1 (PTPN1) is the founding member of the protein tyrosine phosphatase (PTP) family. PTPN1 catalyze the hydrolysis of the phosphate monoesters specifically from tyrosine residues. PTPN1 is known to be involved in signaling pathways that regulate a variety of cellular processes including cell growth and oncogenic transformation. PTPN1 also has been shown to act as a negative regulator of insulin signaling. PTPN1 has also been reported to dephosphorylate epidermal growth factor receptor kinase, which implicated the role of this phosphatase in cell growth control and cell response to interferon stimulation.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 2200 nmol/min/mg
• Molecular Mass: ~71 kDa
• Protein length: Full length
• Purity: >85%
• Applications: Phosphatase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: PTPN1 protein tyrosine phosphatase, non-receptor type 1 [ Homo sapiens ]
• Official Symbol: PTPN1
• Synonyms: PTPN1; protein tyrosine phosphatase, non-receptor type 1; PTP1B; tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase 1B; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, placental
• Gene ID: 5770
• mRNA Refseq: NM_002827
• Protein Refseq: NP_002818
• MIM: 176885
• UniProt ID: P18031
• Chromosome Location: 20q13.1-q13.2
• Pathway: Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Calcineurin-regulated NFAT-dependent transcription in lymphocytes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; EGF receptor (ErbB1) signaling pathway, organism-specific biosystem; Growth hormone receptor signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem
• Function: ephrin receptor binding; hydrolase activity; insulin receptor binding; protein binding; protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; zinc ion binding