Cat.No. :	PDE3B-1526H
Product Overview :	Recombinant human PDE3B (654-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description :	PDE3B is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP and cGMP. PDE3B has high affinity for cAMP and is inhibited by cGMP as well as specific inhibitors such as cilostamide and milrinone. PDE3B is phosphorylated and activated in response to insulin and agents that increase cAMP. Leptin can increase the activity of PDE3B in the hypothalamus leading to a decrease in cAMP concentration. PDE3B-knockout mice exhibited multiple alterations in regulation of lipolysis, lipogenesis, and insulin secretion, as well as signs of peripheral insulin resistance.
Source :	Sf9 Cells
Species :	Human
Tag :	GST
Form :	Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Bio-activity :	156 nmol/min/mg
Molecular Mass :	~85 kDa
Protein length :	654 aa-end
Purity :	>70%
Applications :	PDE Assay
Storage :	Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
Concentration :	0.1μg/μl

Gene Name :	PDE3B phosphodiesterase 3B, cGMP-inhibited [ Homo sapiens ]
Official Symbol :	PDE3B
Synonyms :	PDE3B; phosphodiesterase 3B, cGMP-inhibited; cGMP-inhibited 3,5-cyclic phosphodiesterase B; HcGIP1; CGIP1; CGI-PDE B; cyclic nucleotide phosphodiesterase; cyclic GMP-inhibited phosphodiesterase B; cGIPDE1;
Gene ID :	5140
mRNA Refseq :	NM_000922
Protein Refseq :	NP_000913
MIM :	602047
UniProt ID :	Q13370
Chromosome Location :	11p15.2
Pathway :	Class IB PI3K non-lipid kinase events, organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Hemostasis, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem;
Function :	3,5-cyclic-AMP phosphodiesterase activity; 3,5-cyclic-nucleotide phosphodiesterase activity; cAMP binding; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; drug binding; hydrolase activity; metal ion binding; phosphoprotein binding; phosphoric diester hydrolase activity; protein binding; protein kinase B binding;

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