Recombinant Human TAF1, GST-tagged

• Cat.No.: TAF1-6954H
• Product Overview: Recombinant human TAF1 (1032-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: TAF1 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. TAF1 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. TAF1 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition (1). TAF1 also serves to link the control of transcription to the cell cycle.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutahione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~155 kDa
• Purity: The purity of TAF1 was determined to be >70% by densitometry.
• Applications: Western Blot
• Stability: 1 yr At -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: TAF1 TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa [ Homo sapiens (human) ]
• Official Symbol: TAF1
• Synonyms: TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; transcription initiation factor TFIID subunit 1; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S; NP_004597.2; EC 2.7.11.1; NP_620278.1
• Gene ID: 6872
• mRNA Refseq: NM_138923
• Protein Refseq: NP_620278
• MIM: 313650
• UniProt ID: P21675
• Chromosome Location: Xq13.1
• Pathway: Basal transcription factors; RNA Polymerase II HIV Promoter Escape; RNA Polymerase II Transcription
• Function: TBP-class protein binding; histone acetyl-lysine binding; protein serine/threonine kinase activity