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Recombinant Human TAF1, GST-tagged

Cat.No.: TAF1-6954H
Product Overview: Recombinant human TAF1 (1032-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Description: TAF1 is a DNA-binding protein complex required for RNA polymerase II-mediated transcription of many, if not all, protein-encoding genes in eukaryotic cells. TAF1 plays a key role in the initiation process, since it binds to the TATA element to form a complex that nucleates the assembly of the other components into a preinitiation complex and that may be stable through multiple rounds of transcription. TAF1 may be targeted to specific chromatin-bound promoters and may play a key role in chromatin recognition (1). TAF1 also serves to link the control of transcription to the cell cycle.
Source: Sf9 insect cells using baculovirus
Species: Human
Tag: GST
Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutahione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Molecular Mass: ~155 kDa
Purity: The purity of TAF1 was determined to be >70% by densitometry.
Applications: Western Blot
Stability: 1 yr At -70oC from date of shipment.
Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration: 0.1 ug/ul
Gene Name: TAF1 TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa [ Homo sapiens (human) ]
Official Symbol: TAF1
Synonyms: TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250; TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa; transcription initiation factor TFIID subunit 1; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S; NP_004597.2; EC 2.7.11.1; NP_620278.1
Gene ID: 6872
mRNA Refseq: NM_138923
Protein Refseq: NP_620278
MIM: 313650
UniProt ID: P21675
Chromosome Location: Xq13.1
Pathway: Basal transcription factors; RNA Polymerase II HIV Promoter Escape; RNA Polymerase II Transcription
Function: TBP-class protein binding; histone acetyl-lysine binding; protein serine/threonine kinase activity

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