Recombinant Mouse LMTK3, GST-tagged

• Cat.No.: LMTK3-7004M
• Product Overview: Recombinant mouse LMTK3 (63-484) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: LMTK3, or Lemur tyrosine kinase 3, belongs to the LMR subfamily of tyr protein kinases. LMTK3 decreases the activity of protein kinase C (PKC) and also leads to phosphorylation of AKT (Ser473), thereby increasing binding of forkhead box O3 (FOXO3) to the ESR1 promoter. LMTK3 phosphorylates ERα, protecting it from proteasomal degradation in vitro. Knockdown of LMTK3 leads to a reduction of tumor volume in an orthotopic mouse model and abrogates proliferation of ERα+ but not ERα- cells, indicative of its role in ERα activity. These findings reveal LMTK3 as a new therapeutic target for breast cancer.
• Source: Sf9 Cells
• Species: Mouse
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~75 kDa
• Protein length: 63-484 aa
• Purity: >75%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: Lmtk3 lemur tyrosine kinase 3 [ Mus musculus ]
• Official Symbol: LMTK3
• Synonyms: LMTK3; lemur tyrosine kinase 3; serine/threonine-protein kinase LMTK3; apoptosis-associated tyrosine kinase 3; aatyk3; BC059845; MGC90743
• Gene ID: 381983
• mRNA Refseq: NM_001005511
• Protein Refseq: NP_001005511
• Function: ATP binding; kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity; transferase activity, transferring phosphorus-containing groups