Recombinant Human POLR2E, His-tagged

• Cat.No.: POLR2E-7061H
• Product Overview: Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl,10% glycerol, 1mM DTT
• Molecular Mass: 27.1kDa (233aa)
• Protein length: 1-210aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDDEEET YRLWKIRKTI MQLCHDRGYL VTQDELDQTL EEFKAQFGDK PSEGRPRRTD LTVLVAHNDD PTDQMFVFFP EEPKVGIKTI KVYCQRMQEE NITRALIVVQ QGMTPSAKQS LVDMAPKYIL EQFLQQELLI NITEHELVPE HVVMTKEEVT ELLARYKLRE NQLPRIQAGD PVARYFGIKR GQVVKIIRPS ETAGRYITYR LVQ
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml

Gene Information

• Gene Name: POLR2E polymerase (RNA) II (DNA directed) polypeptide E, 25kDa [ Homo sapiens ]
• Official Symbol: POLR2E
• Synonyms: POLR2E; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; polymerase (RNA) II (DNA directed) polypeptide E (25kD); DNA-directed RNA polymerases I, II, and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; hRPB25; hsRPB5; RPABC1; RPB5; XAP4; RPB5 homolog; DNA-directed RNA polymerase II subunit E; RNA polymerases I, II, and III subunit ABC1; DNA-directed RNA polymerase II 23 kDa polypeptide
• Gene ID: 5434
• mRNA Refseq: NM_002695
• Protein Refseq: NP_002686
• MIM: 180664
• UniProt ID: P19388
• Chromosome Location: 19p13.3
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem
• Function: DNA binding; DNA-directed RNA polymerase activity; protein binding; contributes_to protein kinase activity