||Recombinant Human Ezrin,encoding the mature form of human EZR(Pro2-Leu586) was expressed with a his tag at the N-terminus.
||The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
||The recombinant human EZR consists of 601 amino acids and predicts a molecular mass of 71.2 KDa. It migrates as an approximately 82 KDa band in SDS-PAGE under reducing conditions.
|Predicted N terminal:
||Lyophilized from a 0.2μm filtered solution of PBS 100mM Arg 10% glycerol, 0.1%Twen 20, pH 7.5.
||Greater than 60% as determined by SDS-PAGE
||Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.