Recombinant Human ATP5D, His-tagged

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Recombinant Human ATP5D, His-tagged

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Cat. No. : ATP5D-3572H
Product Overview : Recombinant human ATP5D protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (23-168aa).
Description : ATP5D, also known as F-ATPase delta subunit, catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This protein is the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl
Molecular Weight : 17.3 kDa (167aa), confirmed by MALDI-TOF
Purity : > 95% by SDS - PAGE
Concentration : 0.25 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MAEAAAAPAA ASGPNQMSFT FASPTQVFFN GANVRQVDVP TLTGAFGILA AHVPTLQVLR PGLVVVHAED GTTSKYFVSS GSIAVNADSS VQLLAEEAVT LDMLDLGAAK ANLEKAQAEL VGTADEATRA EIQIRIEANE ALVKALE
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1e79. :

Gene Name : ATP5D ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit [ Homo sapiens ]
Official Symbol : ATP5D
Synonyms : ATP5D; ATP synthase subunit delta, mitochondrial; F-ATPase delta subunit; mitochondrial ATP synthase, delta subunit; mitochondrial ATP synthase complex delta-subunit precursor; EC 3.6.1.14; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit
Gene ID : 513
mRNA Refseq : NM_001001975
Protein Refseq : NP_001001975
MIM : 603150
UniProt ID : P30049
Chromosome Location : 19p13.3
Function : contributes_to ADP binding; contributes_to ATP binding; contributes_to ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; protein complex binding; proton-transporting ATPase activity, rotational mechanism; transmembrane transporter activity; transporter activity

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