||Recombinant human RAB14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-215aa).
||RAB14 is a member of the RAB protein family, low molecular mass monomeric GTPases localized on the cytoplasmic surfaces of distinct membrane bound organelles. This protein is thought to be involved in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus. It is involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT
||26.3 kDa(238aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MGSMATAPYN YSYIFKYIII GDMGVGKSCL LHQFTEKKFM ADCPHTIGVE FGTRIIEVSG QKIKLQIWDT AGQERFRAVT RSYYRGAAGA LMVYDITRRS TYNHLSSWLT DARNLTNPNT VIILIGNKAD LEAQRDVTYE EAKQFAEENG LLFLEASAKT GENVEDAFLE AAKKIYQNIQ DGSLDLNAAE SGVQHKPSAP QGGRLTSEPQ PQREGCGC
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.