Active Recombinant Human TEK protein(Gln771-Ala1124), His&GST-tagged

• Cat.No.: TEK-7296H
• Product Overview: Recombinant Human TEK (NP_000450) (Gln771-Ala1124) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Gln771-Ala1124
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human TEK (aa 771-1124) at 2 μg/ml (100 μl/well) can bind human Ang2-Fc with a linear range of 0.31-20 μg/ml.
• Molecular Mass: The recombinant human TEK /GST chimera consists of 591 amino acids and has a calculated molecular mass of 68.3 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TEK TEK tyrosine kinase, endothelial [ Homo sapiens ]
• Official Symbol: TEK
• Synonyms: TEK; TEK tyrosine kinase, endothelial; venous malformations, multiple cutaneous and mucosal , VMCM; angiopoietin-1 receptor; CD202b; TIE 2; TIE2; VMCM1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2; VMCM; TIE-2; CD202B
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763