"Cxcl12" Related Products


Recombinant Rat SDF-1 alpha

Cat. No.: Cxcl12-65R
Product Overview: Recombinant Rat SDF-1 alpha produced in E. coli is a single, non-glycosylated polypeptide chain containing 68 amino acids and its molecular weight is 7.9 kDa.
Description: SDF-1α and SDF-1β, members of the chemokine α subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1α and SDF-1β cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1α and SDF-1β are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1β and absent from SDF-1α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils.
Source: Escherichia coli.
Quantity: 2ug/10ug/1000μg
Purity: >97% by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-100.0 ng/ml.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
Endotoxin: Less than 1EU/μg of rRtSDF-1a/CXCL12 as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Gene Name: Cxcl12 chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) [ Rattus norvegicus ]
Synonyms: Cxcl12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Sdf1; Cxcl12; SDF-1 gamma; Stromal cell-derived factor 1; stromal cell-derived factor-1 gamma
Gene ID: 24772
mRNA Refseq: NM_001033882
Protein Refseq: NP_001029054
Chromosome Location: 4q42.1
Pathway: Axon guidance; Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration
Function: chemokine activity

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