Recombinant Human Nodal

• Cat.No.: NODAL-30H
• Product Overview: Recombinant Mouse Nodal (His238Leu347), fused with an Nterminal Met, was expressed in E. coli.

Specification

• Cat. No.: NODAL-30H
• Description: Nodal is a 13 kDa member of the TGF­β superfamily of molecules. Its name derives from the fact that it is expressed in the primitive node of the developing embryo. In human, it is synthesized as a 347 amino acid (aa) preproprecursor that contains a 26 aa signal sequence, a 211 aa prodomain, and a 110 aa mature region. Consistent with its TGF­β superfamily membership, it exists as a disulfide­linked homodimer and would be expected to demonstrate a cysteine­knot motif. Mature human Nodal is 99%, 98%, 96% and 98% aa identical to mature canine, rat, bovine and mouse Nodal, respectively. Nodal signals through two receptor complexes, both of which contain members of the TGF­β family of Ser/Thr kinase receptors. The first receptor complex is composed of ActRIB/ALK4 and ActRIIB. Nodal interaction with the ligand­binding subunit ALK4 requires the participation of fucosylated Cripto, a member of the EGF­CFC gene family. The second complex is composed of ALK7 and ActRIIB, and does not require Cripto interaction.
• Source: E. coli
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Predicted MolecularMass: 12.9 kDa (monomer)
• Predicted N terminal: Met
• Formulation: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97% as determined by SDS­PAGE.
• Storage: 1 year from date of receipt, ­20 to ­70 °C as supplied. Avoid repeatedfreeze/thaw cycles.
• OfficialSymbol: NODAL

Gene Information

• Gene Name: NODAL nodal homolog (mouse) [ Homo sapiens ]
• Synonyms: NODAL; nodal homolog (mouse); nodal, mouse, homolog; nodal homolog
• Gene ID: 4838
• mRNA Refseq: NM_018055
• Protein Refseq: NP_060525
• MIM: 601265
• UniProt ID: Q96S42
• Chromosome Location: 10q22.1
• Pathway: Developmental Biology; Regulation of Signaling by NODAL; Signaling by NODAL; TGF-beta signaling pathway
• Function: cytokine activity; growth factor activity; morphogen activity; receptor agonist activity; type I activin receptor binding