||Recombinant Human F3 produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 219 amino acids of the extracellular domain (33-251) having a molecular mass of 29.39 kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The Tissue factor is purified by proprietary chromatographic techniques.
||This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.
||Sterile Filtered clear solution.
||Tissue factor protein is supplied in 22mM Tris.HCl pH8.0, 1mM EDTA and 50% glycerol.
||Greater than 95.0% as determined by SDS-PAGE.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.