Active Recombinant Human CA3 protein, His-tagged

• Cat.No.: CA3-1143H
• Product Overview: Recombinant Human CA3 protein(NP_005172.1)(Met1-Lys260), fused with His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its esterase activity.The specific activity is >5 pmoles/min/μg.
• Molecular Mass: The recombinant human CA3 consisting of 266 amino acids and has a calculated molecular mass of 30.4 kDa as estimated in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Lys260
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ]
• Official Symbol: CA3
• Synonyms: CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434
• Gene ID: 761
• mRNA Refseq: NM_005181
• Protein Refseq: NP_005172
• MIM: 114750
• UniProt ID: P07451