Recombinant Human CA3 protein, His-tagged CA3-1143H

Recombinant Human CA3 protein, His-tagged

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Recombinant Human CA3 protein, His-tagged

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Cat.No. : CA3-1143H
Product Overview : Recombinant Human CA3 (Met1-Lys260) protein was fused to His-tag at C-terminus and expressed in E.coli.
Description : Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.
Source : E.coli
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 30.4 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Lys260
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CA3-1143H :

Human CA3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : CA3
Official Symbol : CA3
Synonyms : CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434
Gene ID : 761
mRNA Refseq : NM_005181
Protein Refseq : NP_005172
MIM : 114750
UniProt ID : P07451

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