Recombinant Human CA3 protein, His-tagged
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|Product Overview :||Recombinant Human CA3 (Met1-Lys260) protein was fused to His-tag at C-terminus and expressed in E.coli.|
|Description :||Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.|
|Predicted N Terminal :||Met1|
|Form :||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 30.4 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE).|
|Protein length :||Met1-Lys260|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CA3-1143H :||
Human CA3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||CA3|
|Official Symbol :||CA3|
|Synonyms :||CA3; carbonic anhydrase III, muscle specific; carbonic anhydrase 3; CAIII; Car3; CA-III; carbonate dehydratase III; FLJ36434|
|Gene ID :||761|
|mRNA Refseq :||NM_005181|
|Protein Refseq :||NP_005172|
|UniProt ID :||P07451|
◆ Recombinant Protein
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|CAR3-2719M||Recombinant Mouse CAR3 Protein||+Inquiry|
|CAR3-1132R||Recombinant Rat CAR3 Protein||+Inquiry|
|CA3-7914HCL||Recombinant Human CA3 293 Cell Lysate||+Inquiry|
◆ Assay kits
|Kit-1937||CAR3 (human) Reporter Assay Kit||+Inquiry|