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Recombinant Human carbonic anhydrase III, muscle specific, His-tagged

Cat.No. : CA3-35H
Product Overview : Recombinant Human Carbonic anhydrase III produced inE.Coli, and having a molecular mass of 33.9 kDa. CAIII is expressed with an amino-terminal hexahistidine tag. The CA-III is purified by proprietary chromatographic techniques.
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Cat. No. : CA3-35H
Description : Carbonic anhydrase (carbonate dehydratase) is a family of metalloenzymes (enzymes that contain one or more metal atoms as a functional component of the enzyme) that catalyze the rapid (and reversible) conversion of carbon dioxide to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. Carbonic anhydrase greatly increases the rate of the reaction, with typical catalytic rates of the different forms of this enzyme ranging between 104 and 106 reactions per second. The active site of most carbonic anhydrases contains a zinc ion. CAIII is a cytoplasmic isoenzyme, but is released into the circulation following injury.
Source : Escherichia Coli.
Physical Appearance : Sterile Filtered blue solution.
Purity : Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : Supplied in 1x Laemmli Buffer: 25 mM Tris-HCl pH6.8; 50 mM DTT; 1% SDS; 0.1% Bromophenol Blue and 2.5% Glycerol.
Stability : CAIII although stable at 14°C for 1 week; should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Publication :
Local pH Gradient Initiated by Light on TiO2 for Light-Triggered Modulation of Polyhistidine-Tagged Proteins (2016)
Gene Name : CA3 carbonic anhydrase III, muscle specific [ Homo sapiens ]
Synonyms : CA3; carbonic anhydrase III, muscle specific; Car3; CAIII;Carbonic anhydrase 3;CA-III; Carbonate dehydratase III; Carbonic anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1
Gene ID : 761
mRNA Refseq : NM_005181
Protein Refseq : NP_005172
UniProt ID : P07451
Chromosome Location : 8q13-q22
MIM : 114750
Pathway : Nitrogen metabolism
Function : carbonate dehydratase activity; lyase activity; metal ion binding; nickel ion binding; zinc ion binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which tissues is CA3 expression most prominently observed? 10/22/2022

CA3 is primarily found in skeletal muscles.

What post-translational modifications are characteristic of CA3? 06/23/2022

Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.

Are there known inhibitors that specifically target CA3? 09/19/2021

Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.

How does CA3 participate in metabolic processes within the cell? 02/04/2021

CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.

How does CA3 enzymatic activity differ from other carbonic anhydrase isoforms? 10/26/2019

While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.

Are there any pathological conditions directly linked to CA3 dysregulation? 06/08/2017

CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.

What is the primary physiological role of CA3 in cells? 05/26/2017

CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.

Customer Reviews (3)

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Reviews
09/23/2022

    Outstanding protein sequencing capabilities, unrivaled accuracy.

    05/23/2022

      Precise purification techniques, yield exceptional samples.

      05/25/2021

        Efficient sample handling, optimizes our laboratory workflow.

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