Recombinant Human Cyclin A2, GST-tagged


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Recombinant Human Cyclin A2, GST-tagged

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Cat. No. : CCNA2-256H
Product Overview : Human CCNA2 (NM_001237) full-length recombinant protein with GST tag expressed in SF9 cells.
Description : The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
Theoretical MW : 58, 78
Preparation Method : Insect cell (SF9) expression system
Purity : > 70% by densitometry
Activity : The specific activity of was determined to be 822 nmol /min/mg as per Activity Assay Protocol.
Application : SDS-PAGE
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
Storage : Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Unitprot ID : P20248
PDB rendering based on1e9h. :

Gene Name : CCNA2 cyclin A2 [ Homo sapiens ]
Official Symbol : CCNA2
Synonyms : CCNA; CCNA2; CCN1; cyclin-A2; cyclin-A; OTTHUMP00000164087
Gene ID : 890
mRNA Refseq : NM_001237
Protein Refseq : NP_001228
MIM : 123835
Chromosome Location : 4q25-q31
Pathway : APC/C-mediated degradation of cell cycle proteins; ATF-2 transcription factor network; B Cell Receptor Signaling Pathway; Cell Cycle, Mitotic; Cell cycle; Cyclin A/B1 associated events during G2/M transition; Cyclin A:Cdk2-associated events at S phase entry; DNA Replication; E2F transcription factor network; G1/S Transition; G2/M Transition; IL2 signaling events mediated by STAT5; Id Signaling Pathway; Orc1 removal from chromatin; Progesterone-mediated oocyte maturation; Regulation of APC/C activators between G1/S and early anaphase
Function : protein binding; protein kinase binding

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