Recombinant Human CDKN1B, His-tagged CDKN1B-4729H

Recombinant Human CDKN1B, His-tagged

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Recombinant Human CDKN1B, His-tagged

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Cat. No. : CDKN1B-4729H
Product Overview : Recombinant human CDKN1B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description : CDKN1B, also known as cyclin-dependent kinase inhibitor 1B, belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. This protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
Molecular Weight : 24.2 kDa (218aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
Purity : > 90% by SDS - PAGE
Concentration : 0.25 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MSNVRVSNGS PSLERMDARQ AEHPKPSACR NLFGPVDHEE LTRDLEKHCR DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK VPAQESQDVS GSRPAAPLIG APANSEDTHL VDPKTDPSDS QTGLAEQCAG IRKRPATDDS STQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Cell cycle; Chronic myeloid leukemia; ErbB signaling pathway; Pathways in cancer; Prostate cancer; Small cell lung cancer
PDB rendering based on 1jsu :

Gene Name : CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) [ Homo sapiens ]
Official Symbol : CDKN1B
Synonyms : CDKN1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN4; CDKN4; MEN1B; P27KIP1; cyclin -dependent kinase inhibitor 1B; p27Kip1; Cyclin-dependent kinase inhibitor p27
Gene ID : 1027
mRNA Refseq : NM_004064
Protein Refseq : NP_004055
MIM : 600778
UniProt ID : P46527
Chromosome Location : 12p13.1-p12
Function : cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase inhibitor activity; transforming growth factor beta receptor, cytoplasmic mediator activity

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