Recombinant Human CDKN1B Protein, DDK-tagged

• Cat.No.: CDKN1B-006H
• Product Overview: Recombinant protein of full length human cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B), with C-terminal DDK tag, was expressed in sf9.

Specification

• Description: This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
• Source: Insect cell
• Species: Human
• Tag: DDK
• Molecular Mass: 22.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 50mM Tris-HCl, pH 8.0, 100mM glycine, 10% glycerol.

Gene Information

• Gene Name: CDKN1B cyclin-dependent kinase inhibitor 1B (p27, Kip1) [ Homo sapiens ]
• Official Symbol: CDKN1B
• Synonyms: CDKN1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; KIP1; P27KIP1; MEN4; CDKN4; MEN1B
• Gene ID: 1027
• mRNA Refseq: NM_004064
• Protein Refseq: NP_004055
• MIM: 600778
• UniProt ID: P46527