Active Recombinant Human DAPK1 protein, His&GST-tagged

• Cat.No.: DAPK1-971H
• Product Overview: Recombinant Human DAPK1 protein(P53355-1)(Met1-Leu363), fused with His&GST tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glyPlease contact us for any concerns or special requirements.Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
• Molecular Mass: The recombinant human DAPK1 (aa 1-363)/GST chimera consists of 600 amino acids and has a calculated molecular mass of 69.4 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Leu363
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DAPK1 death-associated protein kinase 1 [ Homo sapiens ]
• Official Symbol: DAPK1
• Synonyms: DAPK1; death-associated protein kinase 1; DAPK; DAP kinase 1; DKFZp781I035
• Gene ID: 1612
• mRNA Refseq: NM_004938
• Protein Refseq: NP_004929
• MIM: 600831
• UniProt ID: P53355