||Recombinant human DNAJB8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||DNAJB8, also known as dnaJ homolog subfamily B member 8, is superior suppressors of aggregation and toxicity of disease-associated polyglutamine proteins. DNAJB8 is (de)acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
||27.8 kDa (252aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.