Recombinant Human Glycine Cleavage System Protein H (Aminomethyl Carrier), His-tagged GCSH-1281H

Recombinant Human Glycine Cleavage System Protein H (Aminomethyl Carrier), His-tagged

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Recombinant Human Glycine Cleavage System Protein H (Aminomethyl Carrier), His-tagged

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Cat. No. : GCSH-1281H
Product Overview : Recombinant human GCSH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description : The enzyme system for cleavage of glycine (glycine cleavage system) is composed of four mitochondrial protein components: P protein, H protein, T protein, and L protein. GCSH is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Form : Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.
Molecular Weight : 16.4 kDa (149aa), confirmed by MALDI-TOF
Purity : > 95 % by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MGSMSVRKFT EKHEWVTTEN GIGTVGISNF AQEALGDVVY CSLPEVGTKL NKQDEFGALE SVKAASELYS PLSGEVTEIN EALAENPGLV NKSCYEDGWL IKMTLSNPSE LDELMSEEAY EKYIKSIEE
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : GCSH glycine cleavage system protein H (aminomethyl carrier) [ Homo sapiens ]
Official Symbol : GCSH
Synonyms : GCSH; glycine cleavage system protein H (aminomethyl carrier); GCE; NKH; glycine cleavage system H protein, mitochondrial; OTTHUMP00000174971; lipoic acid-containing protein; mitochondrial glycine cleavage system H-protein; Glycine cleavage system H protein, mitochondrial
Gene ID : 2653
mRNA Refseq : NM_004483
Protein Refseq : NP_004474
MIM : 238330
UniProt ID : P23434
Chromosome Location : 16q23.2
Function : aminomethyltransferase activity; enzyme binding

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