Active Recombinant Human HMGB1 protein, His-tagged

• Cat.No.: HMGB1-3827H
• Product Overview: Recombinant Human HMGB1 protein(NP_002119.1)(Met1-Glu215), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER.The EC50 of human AGER is 0.27 μg/ml.
• Molecular Mass: The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
• Protein Length: Met1-Glu215
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 93 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: HMGB1 high mobility group box 1 [ Homo sapiens ]
• Official Symbol: HMGB1
• Synonyms: HMGB1; high mobility group box 1; high mobility group (nonhistone chromosomal) protein 1 , high mobility group box 1 , HMG1; high mobility group protein B1; Amphoterin; DKFZp686A04236; high mobility group protein 1; HMG3; SBP 1; Sulfoglucuronyl carbohydrate binding protein; HMG-1; high-mobility group box 1; high-mobility group (nonhistone chromosomal) protein 1; HMG1; SBP-1
• Gene ID: 3146
• mRNA Refseq: NM_002128
• Protein Refseq: NP_002119
• MIM: 163905
• UniProt ID: P09429

Reviews

09/22/2021

Broad storage conditions of HMGB1ins make the experimental process more stable and reliable, which provides a strong guarantee for scientific research.

05/02/2020

I found that tHMGB1 exhibited very good stability during storage, which allowed us to perform experiments easily and obtain reliable results.

01/11/2019

The anti-contamination ability of HMGB1 was excellent in Western blot, and the contamination problem in the experiment was avoided.

Q&As

Why is HMGB1 protein associated with tumors?

This protein can promote tumor growth, invasion and angiogenesis, and plays an important role in tumor immune evasion.

Is HMGB1 protein involved in tumor metastasis?

Yes, HMGB1 protein plays an important role in tumor metastasis, particularly by promoting invasion of tumor cells and migration of the hematopoietic system.

What is the association between HMGB1 protein and cardiovascular disease?

Is involved in inflammatory response, vascular damage, and thrombosis in cardiovascular disease and may be associated with myocardial ischemia-reperfusion injury.

What is the effect of HMGB1 protein on the immune system?

HMGB1 protein can promote the activation, chemotaxis and inflammatory response of immune cells, and is also involved in regulating the balance of autoimmune response.

What role does HMGB1 play in diabetes?

HMGB1 protein is associated with processes such as chronic inflammation and insulin resistance in diabetes and may be a potential target for treatment and prevention.

Does HMGB1 play a role in liver disease?

The protein is involved in pathological processes such as liver fibrosis, hepatitis, and liver cancer in liver disease and may be a target for therapeutic strategies.