Recombinant Mouse Il1b protein

• Cat.No.: Il1b-462M
• Product Overview: Recombinant Mouse Il1b protein(P10749) was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 152 amino acids
• Form: Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4, with 5 % trehalose, 0.02 % Tween-20.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/ml, corresponding to a specific activity of > 5.0 × 10^8 IU/mg.
• Molecular Mass: Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
• Endotoxin: Less than 1 EU/μg of rMuIL-1β as determined by LAL method.
• Purity: > 96 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  - 12 months from date of receipt, -20 to -70 °C as supplied.
  - 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.
• AA Sequence: VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS

Gene Information

• Gene Name: Il1b interleukin 1 beta [ Mus musculus ]
• Official Symbol: Il1b
• Synonyms: IL1B; interleukin 1 beta; interleukin-1 beta; IL-1 beta; Il-1b; IL-1beta
• Gene ID: 16176
• mRNA Refseq: NM_008361
• Protein Refseq: NP_032387