Recombinant Human IL1RL1 protein, His-tagged IL1RL1-3991H

Recombinant Human IL1RL1 protein, His-tagged

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Cat.No. : IL1RL1-3991H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human IL1RL1 (Lys19-Phe328) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Lys19
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 36.9 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Lys19-Phe328
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IL1RL1-3991H :

Human IL-1RL1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of IL1RL1-3991H :

Immobilized human IL33 at 5 μg/mL (100 μL/well) can bind Human IL-1RL1, His Tag with a linear range of 0.125-0.5 μg/mL (QC tested).

Gene Name : IL1RL1
Official Symbol : IL1RL1
Synonyms : IL1RL1; interleukin 1 receptor-like 1; interleukin-1 receptor-like 1; DER4; FIT 1; homolog of mouse growth stimulation expressed; IL33R; ST2; ST2L; ST2V; T1; growth stimulation-expressed; interleukin 1 receptor-related protein; homolog of mouse growth stimulation-expressed; FIT-1; MGC32623
Gene ID : 9173
mRNA Refseq : NM_003856
Protein Refseq : NP_003847
MIM : 601203
UniProt ID : Q01638
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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