Active Recombinant Human Integrin, Beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)

• Cat.No.: ITGB1-1484H
• Product Overview: TIMP1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 184 amino acids with a molecular weight of 20.7 kDa though on SDS-PAGE migrates at about 28kDa due to the glycosilation. The TIMP1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: ITGB1-1484H
• Description: TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site.
• Source: Human
• Host: HEK293-F Cells.
• Form: The TIMP1 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.
• Purity: Greater than 95.0% as determined by SDS-PAGE
• Bio-activity: IC50 = 0.4 to 1.2 nM, determined by the concentration dependent inhibition of human MMP-2 hydrolysis of a peptide substrate (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-(3-[2, 4-dinitrophenyl]-L-2, 3-diaminopropionyl)-Ala-Arg-NH2, under conditions in which recombinant pro-MMP-2 is activated, and the peptide substrate concentration is 5µM.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Solubility: It is recommended to reconstitute the lyophilized TIMP1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Amino acid sequence: CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
• Storage: Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: ITGB1 integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) [ Homo sapiens ]
• Official Symbol: ITGB1
• Synonyms: ITGB1; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); FNRB; CD29; GPIIA; Fibronectin receptor subunit beta; MDF2; MSK12; VLA-BETA; VLAB; integrin beta-1; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide; CD29 antigen; VLA-4 subunit beta
• Gene ID: 3688
• mRNA Refseq: NM_002211
• Protein Refseq: NP_002202
• MIM: 135630
• UniProt ID: P05556
• Chromosome Location: 10p11.2
• Pathway: Adaptive Immunity Signaling; Arf6 trafficking events; Arrhythmogenic right ventricular cardiomyopathy (ARVC); Axon guidance; CXCR4-mediated signaling events; Cell junction organization; Dilated cardiomyopathy
• Function: actin binding; alpha-actinin binding; identical protein binding; protein binding; protein domain specific binding; receptor activity