| Cat. No. : |
Lep-261M |
| Source : |
Mouse |
| Host : |
Escherichia coli. |
| Form : |
The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3. |
| Purity : |
Greater than 99.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE. |
| Physical Appearance : |
White lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant in sterile water or sterile 0.4% NaHCO3adjusted to pH 8-9, not less than 100µg/ml, which can then be further diluted with other aqueous solutions. |
| Amino acid sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln Biological ActivityProSpecμs Leptin Antagonist Triple Mutant Mouse Recombinant is capable of inhibiting Leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human Leptin receptor. It also inhibits various Leptin effects in several in vitro bioassays. |
| Protein content : |
Protein quantization was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Storage : |
Lyophilized Leptin Antagonist Triple Mutant Mouse Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 Leptin mutant mg/ml and up to 2 mM and filter sterilization LEP mutant can be stored at 4°C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
