|Product Overview:||Recombinant Human LGALS3(1 - 250 aa) fused with GST tag was expressed in E. coli.|
|Description:||This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
Note: GeneID 3958 had the official symbol LGALS2 and was identified as the human homolog of mouse Mac-2 (macrophage galactose-specific lectin) in M35368.1 and PMID: 2402511. The official symbol for this gene is now LGALS3.
|Form:||Protein lyophilized in sterile PBS (58 mM Na2HPO4, 17 mM NaH2PO4, 68 mM NaCl, 100 mM GSH, pH 8.0). Trehalose (5-8%) and mannitol (5-8%) protectants were added before lyophilization.|
|Protein length:||1 - 250 aa|
|Purity:||75%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Storage:||Store for up to 12 months at -20 centigrade to -80 centigrade as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8 centigrade for two weeks.
Long-term storage: Aliquot and store at -20 centigrade to -80 centigrade for up to 6 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
|Reconstitution:||Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping:||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|Gene Name:||LGALS3 lectin, galactoside-binding, soluble, 3 [ Homo sapiens ]|
|Synonyms:||LGALS3; lectin, galactoside-binding, soluble, 3; LGALS2; galectin-3; galectin 3; GALIG; MAC 2; lectin L-29; 35 kDa lectin; MAC-2 antigen; IgE-binding protein; laminin-binding protein; galactose-specific lectin 3; carbohydrate-binding protein 35; L31; GAL3; MAC2; CBP35; GALBP;|
|Pathway:||Advanced glycosylation endproduct receptor signaling, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem;|
|Function:||IgE binding; protein binding; sugar binding;|