Recombinant Human Nerve Growth Factor (beta polypeptide) NGF-573H

Recombinant Human Nerve Growth Factor (beta polypeptide)


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Recombinant Human Nerve Growth Factor (beta polypeptide)

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Cat. No. : NGF-573H
Product Overview : Human NGF-beta is a noncovalently disulfide-linked homodimer, consisting of 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.
Description : Human NGF-beta is a neurotrophic factor related to BDNF, NT-3 and NT-4. Human NGF-beta acts as a neurotrophic factor through its receptor beta-NGFR, and is involved in the development and sustaining the sensory and sympathetic nervous systems. H-NGFbeta also is also involved in the growth and differentiation of B lymphocytes and B-cell survival.
Species : Human
Source : E.coli
Form : Human NGF-beta is lyophilized with no additives.
Purity : Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Biological activity is determined by the ability to stimulate neurite growth in chick E9 DRG cells. The ED50 is < 1.0 ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 97% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Apoptosis, MAPK signaling pathway, Signalling by NGF
Renderingof NGF from PDB 1SG1. :

Gene Name : NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
Official Symbol : NGF
Synonyms : NGF; nerve growth factor (beta polypeptide); NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428; Beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor, beta polypeptide; beta-nerve growth factor; OTTHUMP00000013653
Gene ID : 4803
mRNA Refseq : NM_002506
Protein Refseq : NP_002497
MIM : 162030
UniProt ID : P01138
Chromosome Location : 1p13.1
Function : growth factor activity, nerve growth factor receptor binding, receptor signaling protein activity

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