||Recombinant human PDGFRB contains C-terminal polyhistidine tag and has a calculated MW of 60.3 KDa. As a result of glycosylation, DTT-reduced protein migrates as 95-100 kDa polypeptide in SDS-PAGE.
||Human platelet-derived growth factor receptor, beta polypeptide (PDGFRB) is receptor that binds specifically to PDGFB and has a tyrosine-protein kinase activity. Is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor (PDGF) family. The PDGFR/PDGF system includes two receptors (PDGFRA and PDGFRB) and four ligands (A, B, C and D). The receptors PDGFRA and PDGFRB are related in sequence and both are members of the class III subtype of receptor tyrosine kinases (RTKs). Other class III RTKs are CSF1R, KIT and FLT3. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the μ and μ receptors is independently regulated in various cell types.
||Recombinant human PDGFRB Met1-Phe530 (Accession NP_002600) was produced in human HEK293 cells.
||Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
||>95% as determined by SDS-PAGE. All lots are greater than 95% pure.
||Less than 1.0 EU per 1 μg of the human PDGFRB by the LAL method.
||Store at -70°C upon received. Avoid repeated freeze-thaw cycles.