Recombinant Human SOD1 protein, His-tagged SOD1-1161H

Recombinant Human SOD1 protein, His-tagged

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Cat.No. : SOD1-1161H Optional Service: Optional requirements on this protein
Product Overview : Recombinant Human SOD1 (Ala2-Gln154) protein was fused to His-tag at N-terminus and expressed in E.coli.
Description : Superoxide dismutase [Cu-Zn] (SOD1) is also known as superoxide dismutase 1 (hSod1), an enzyme that in humans is encoded by the SOD1 gene, located on chromosome 21. SOD1 can bind copper and zinc ions and is one of three superoxide dismutases responsible for destroying free superoxide radicals in the body. The encoded isozyme (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein, acting as a homodimer to convert naturally occurring, but harmful, superoxide radicals to molecular oxygen and hydrogen peroxide. Furthermore, the mutations of SOD1 gene can result in a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord.
Source : E.coli
Species : Human
Tag : His
Predicted N Terminal : Ala2
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 16.8 kDa. The protein migrates as 20 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Ala2-Gln154
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >97% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of SOD1-1161H :

Human SOD1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 97%.

Gene Name : SOD1
Official Symbol : SOD1
Synonyms : SOD1; superoxide dismutase 1, soluble; ALS, ALS1, amyotrophic lateral sclerosis 1 (adult); superoxide dismutase [Cu-Zn]; IPOA; SOD, soluble; indophenoloxidase A; Cu/Zn superoxide dismutase; superoxide dismutase, cystolic; ALS; SOD; ALS1; hSod1; homodimer
Gene ID : 6647
mRNA Refseq : NM_000454
Protein Refseq : NP_000445
MIM : 147450
UniProt ID : P00441
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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