Recombinant Human SPAM1, His-Fc-tagged

• Cat.No.: SPAM1-1532H
• Product Overview: Recombinant human PH20 is produced in CHO cells. By combining the sequence of PH20 with the Fc fragment of hunman IgG or 6His

Specification

• Cat. No.: SPAM1-1532H
• Description: Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Human
• Host: Chinese Hamster Ovary
• Purity: >90%
• Predicted N terminal: LNFRAPPVIP
• Molecular Mass: Containing 6-His tag, length 488aa, weigth 62kDa; PH20 contains 482 amino acid residues, and 670aa after combining with the Fc fragment of IgGII, the MW of monomer and dimer is about 91 kDa and 182 kDa, respectively.
• Storage: 4μ for 6 months; -20μ for 1 year. Avoid repeated freezing and thawing.

Gene Information

• Gene Name: SPAM1 sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) [ Homo sapiens ]
• Official Symbol: SPAM1
• Synonyms: SPAM1; HYA1; PH20; HYAL1; HYAL3; HYAL5; PH-20; SPAG15; MGC26532; hyaluronidase PH-20; hyal-PH20; OTTHUMP00000211901; OTTHUMP00000211902; OTTHUMP00000211952; sperm surface protein PH-20; hyaluronoglucosaminidase PH-20
• Gene ID: 6677
• mRNA Refseq: NM_001174044
• Protein Refseq: NP_001167515
• MIM: 600930
• UniProt ID: P38567
• Chromosome Location: 7q31.3
• Pathway: Glycosaminoglycan degradation; Metabolic pathways
• Function: catalytic activity; hyalurononglucosaminidase activity; hydrolase activity, acting on glycosyl bonds