Active Recombinant Human Tumor Necrosis Factor (Ligand) Superfamily, Member 10

• Cat.No.: TNFSF10-4752H
• Product Overview: Recombinant Human soluble Trail also called TNF-related apoptosis-inducing Ligand, TNFSF10, Apo2 Ligand AND TL2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 168 amino acids and having a molecular mass of 19.6 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein functioning as a ligand that induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253).
• Form: Lyophilized from a concentrated solution (1mg/ml) contains 150 mM NaCl, and 50mM sodium phosphate, pH 7.4.
• Purity: Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Bio-activity: Recombinant Human TRAIL is fully biologically active when compared to standard. The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensetive U343MG cells, ED50 for this effect is 1-3 ng/ml.
• Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
• Solubility: Lyophilized rHuTRAIL although stable at room temperature for 3 weeks, should be stored desiccated below -18μ. Upon reconstitution rHuTRAIL should be stored at 4μ between 2-7 days and for future use below -18μ. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
• Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
• Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
• Endotoxin Level: Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
• Amino acid sequence: MRERGPQRVA AHITGTRGRS NTLSSPNSKN EKALGRKINS WESSRSGHSF LSNLHLRNGE LVIHEKGFYY IYSQTYFRFQ EEIKENTKND KQMVQYIYKY TSYPDPILLM KSARNSCWSK DAEYGLYSIY QGGIFELKEN DRIFVSVTNE HLIDMDHEAS FFGAFLVG
• Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

Gene Information

• Gene Name: TNFSF10 tumor necrosis factor (ligand) superfamily, member 10 [ Homo sapiens ]
• Official Symbol: TNFSF10
• Synonyms: TNFSF10; tumor necrosis factor (ligand) superfamily, member 10; TRAIL; Apo-2L; TL2; CD253; Tumor necrosis factor ligand superfamily member 10; TNF-related apoptosis-inducing ligand; Protein TRAIL; Apo-2 ligand; APO2L; TNF-related apoptosis inducing ligand TRAIL; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; CD253 antigen; tumor necrosis factor apoptosis-inducing ligand splice variant delta
• Gene ID: 8743
• mRNA Refseq: NM_003810
• Protein Refseq: NP_003801
• MIM: 603598
• UniProt ID: P50591
• Chromosome Location: 3q26
• Pathway: Apoptosis; Cytokine-cytokine receptor interaction; Natural killer cell mediated cytotoxicity
• Function: cytokine activity; metal ion binding; tumor necrosis factor receptor binding; zinc ion binding