|Cat. No. :
|Product Overview :
||Recombinant human UPK3A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
||UPK3A belongs to the uroplakin-3 family. Uroplakins (UP) are key components of the urothelium. They are transmembrane proteins that pass the lipid bilayer once (UPII, UPIIIa, and UPIIIb) or four times (UPIa and UPIb; both belonging to the "tetraspanin" family). All have large luminal/extracellular domains, but only UPIIIa and UPIIIb have significant cytoplasmic portions in their C-termini. UPK3A is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinomas. Mutations in this gene may be associated with renal adysplasia
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 150mM NaCl
|Molecular Weight :
||23.1kDa (214aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25mg/ml (determined by Bradford assay)
|Sequences of amino acids :
||MGSSHHHHHH SSGLVPRGSH MGSHMVNLQP QLASVTFATN NPTLTTVALE KPLCMFDSKE ALTGTHEVYL YVLVDSAISR NASVQDSTNT PLGSTFLQTE GGRTGPYKAV AFDLIPCSDL PSLDAIGDVS KASQILNAYL VRVGANGTCL WDPNFQGLCN PPLSAATEYR FKYVLVNMST GLVEDQTLWS DPIRTNQLTP YSTIDTWPGR RSGG
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.