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Recombinant Mouse YWHAE, GST-tagged

Cat. No.: YWHAE-1193H
Product Overview: A DNA sequence encoding the mouse YWHAE ( P62259 ) ( Met 1 – Gln 255 ) was fused with the GST tag at the N-terminus
Description: Mouse tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, epsilon polypeptide ( YWHAE ), is a member of the14- 3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner.
Source: Human
Host: E.coli
Form: Lyophilized from sterile 50 mM Tris, 2 mM GSH, 1 mM DTT, pH 8.2 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Purity: > 90 % as determined by SDS-PAGE
Endotoxin: ﹤1.0 EU per μg protein as determined by the LAL method
Stability: Samples are stable for up to twelve months from date of receipt at -70°C
Predicted N terminal: Met
Molecular Mass: The recombinant mouse YWHAE / GST chimera consists of 487 amino acids and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions
Storage: Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
Pathways: Alpha6-Beta4 Integrin Signaling Pathway; Calcium Regulation in the Cardiac Cell; Centrosome maturation

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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