| Cat. No. : |
YWHAE-1193H |
| Description : |
Mouse tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, epsilon polypeptide ( YWHAE ), is a member of the14- 3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. |
| Source : |
Human |
| Host : |
E.coli |
| Form : |
Lyophilized from sterile 50 mM Tris, 2 mM GSH, 1 mM DTT, pH 8.2 Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Purity : |
> 90 % as determined by SDS-PAGE |
| Endotoxin : |
﹤1.0 EU per μg protein as determined by the LAL method |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70°C |
| Predicted N terminal : |
Met |
| Molecular Mass : |
The recombinant mouse YWHAE / GST chimera consists of 487 amino acids and has a predicted molecular mass of 56.1 kDa. It migrates as an approxiamtely 48 kDa band in SDS-PAGE under reducing conditions |
| Storage : |
Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Pathways : |
Alpha6-Beta4 Integrin Signaling Pathway; Calcium Regulation in the Cardiac Cell; Centrosome maturation |
