Recombinant Human LPL, His-tagged

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Recombinant Human LPL, His-tagged

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Cat. No. : LPL-1014H
Product Overview : Total 458 AA. MW: 51.61 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Description : LPL is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires Apo-CII as a cofactor.
Source : E. coli
Species : Human
Form : Filtered (0. 4 μm) and lyophilized from 0.5 mg/ml in 50mM Acetate beffer, pH=4
Research topic : Energy metabolism and body weight regulation
Reconstitution : Add 0.1 M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications : Western blotting
Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL TEST to determine endotoxin level
Sequences of amino acids : MKHHHHHHAS ADQRRDFIDI ESKFALRTPE DTAEDTCHLI PGVAESVATC HFNHSSKTFM VIHGWTVTGM YESWVPKLVA ALYKREPDSN VIVVDWLSRA QEHYPVSAGY TKLVGQDVAR FINWMEEEFN YPLDNVHLLG YSLGAHAAGI AGSLTNKKVN RITGLDPAGP NFEYAEAPSR LSPDDADFVD VLHTFTRGSP GRSIGIQKPV GHVDIYPNGG TFQPGCNIGE AIRVIAERGL GDVDQLVKCS
Storage : Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Gene Name : LPL lipoprotein lipase [ Homo sapiens ]
Official Symbol : LPL
Synonyms : LPL; lipoprotein lipase; LIPD; HDLCQ11; lipoprotein lipase; OTTHUMP00000034806
Gene ID : 4023
mRNA Refseq : NM_000237
Protein Refseq : NP_000228
MIM : 609708
UniProt ID : P06858
Chromosome Location : 8p22
Pathway : Adipogenesis; Alzheimer"s disease; Chylomicron-mediated lipid transport; Diabetes pathways; Fatty Acid Beta Oxidation; Glycerolipid metabolism; Lipid digestion; Lipoprotein metabolism; Metabolism of lipids and lipoproteins; PPAR signaling pathway; Statin Pathway; Transcriptional Regulation of White Adipocyte Differentiation; Triacylglyceride Synthesis; Vitamin A and carotenoid metabolism
Function : heparin binding; hydrolase activity; lipoprotein lipase activity; phospholipase activity; protein binding; receptor binding; triglyceride binding; triglyceride lipase activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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