"LPL" Related Products

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Recombinant Human LPL, His-tagged

Cat. No.: LPL-1014H
Product Overview: Total 458 AA. MW: 51.61 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Description: LPL is a member of the lipase gene family, which includes pancreatic lipase, hepatic lipase, and endothelial lipase. It is a water soluble enzyme that hydrolyzes triglycerides in lipoproteins, such as those found in chylomicrons and very low-density lipoproteins (VLDL), into two free fatty acids and one monoacylglycerol molecule. It is also involved in promoting the cellular uptake of chylomicron remnants, cholesterol-rich lipoproteins, and free fatty acids. LPL requires Apo-CII as a cofactor.
Source: E. coli
Species: Human
Form: Filtered (0. 4 μm) and lyophilized from 0.5 mg/ml in 50mM Acetate beffer, pH=4
Research topic: Energy metabolism and body weight regulation
Reconstitution: Add 0.1 M Acetate buffer pH 4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications: Western blotting
Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL TEST to determine endotoxin level
Sequences of amino acids: MKHHHHHHAS ADQRRDFIDI ESKFALRTPE DTAEDTCHLI PGVAESVATC HFNHSSKTFM VIHGWTVTGM YESWVPKLVA ALYKREPDSN VIVVDWLSRA QEHYPVSAGY TKLVGQDVAR FINWMEEEFN YPLDNVHLLG YSLGAHAAGI AGSLTNKKVN RITGLDPAGP NFEYAEAPSR LSPDDADFVD VLHTFTRGSP GRSIGIQKPV GHVDIYPNGG TFQPGCNIGE AIRVIAERGL GDVDQLVKCS
Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Gene Name: LPL lipoprotein lipase [ Homo sapiens ]
Official Symbol: LPL
Synonyms: LPL; lipoprotein lipase; LIPD; HDLCQ11; lipoprotein lipase; OTTHUMP00000034806
Gene ID: 4023
mRNA Refseq: NM_000237
Protein Refseq: NP_000228
MIM: 609708
UniProt ID: P06858
Chromosome Location: 8p22
Pathway: Adipogenesis; Alzheimer"s disease; Chylomicron-mediated lipid transport; Diabetes pathways; Fatty Acid Beta Oxidation; Glycerolipid metabolism; Lipid digestion; Lipoprotein metabolism; Metabolism of lipids and lipoproteins; PPAR signaling pathway; Statin Pathway; Transcriptional Regulation of White Adipocyte Differentiation; Triacylglyceride Synthesis; Vitamin A and carotenoid metabolism
Function: heparin binding; hydrolase activity; lipoprotein lipase activity; phospholipase activity; protein binding; receptor binding; triglyceride binding; triglyceride lipase activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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