Recombinant Porcine ADIPOQ, Flag-tagged

• Cat.No.: ADIPOQ-218P
• Product Overview: Total 238 AA. MW: 26.0 kDa (calculated). N-Terminal Flag-tag, UniProtKB/TrEMBL Q6PP07 with 13 extra AA (highlighted).

Specification

• Species: Porcine
• Source: HEK293
• Tag: Flag
• Description: This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
• Form: Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 50mM NaCl, pH 7.5.
• Research topic: Energy metabolism and body weight regulation.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: HVDYKDDDDK PAGETTEKPG ALLPMPKGAC AGWMAGIPGH PGHNGTPGRD GRDGVPGEKG EKGDTGLTGP KGDTGESGVT GVEGPRGFPG IPGRKGEPGE SAYVYRSAFS VGLETRVTVP NMPIRFTKIF YNQQNHYDVT TGKFHCNIPG LYYFSFHITV YLKDVKVSLY KKDKAVLFTY DQYQDKNVDQ ASGSVLLYLE KGDQVWLQAY GDEENNGVYA DNVNDSIFTG FLLYHNIE
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
• Pathways: Adipocytokine signaling pathway; Adipogenesis; Diabetes pathways; PPAR signaling pathway; Transcriptional Regulation of White Adipocyte Differentiation; Type II diabetes mellitus

Gene Information

• Gene Name: ADIPOQ adiponectin, C1Q and collagen domain containing [ Homo sapiens ]
• Official Symbol: ADIPOQ
• Synonyms: ADIPOQ; adiponectin, C1Q and collagen domain containingprovided; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1; adiponectin; OTTHUMP00000210047; OTTHUMP00000210048; gelatin-binding protein 28; adipose specific collagen-like factor; 30 kDa adipocyte complement-related protein; adipocyte complement-related 30 kDa protein; adipose most abundant gene transcript 1 protein
• Gene ID: 9370
• mRNA Refseq: NM_004797
• Protein Refseq: NP_004788
• MIM: 605441
• UniProt ID: Q15848
• Chromosome Location: 3q27
• Function: cytokine activity; eukaryotic cell surface binding; hormone activity; identical protein binding; protein binding; protein homodimerization activity; receptor binding