Recombinant Human IL6, His-tagged IL6-323H

Recombinant Human IL6, His-tagged

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Recombinant Human IL6, His-tagged

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Cat. No. : IL6-323H
Product Overview : Total 193 AA. MW: 22.05 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Description : Interleukin-6 is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis, and it may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections.
Source : E. coli
Species : Human
Form : Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5.
Research topic : Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation.
Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications : Western blotting
Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
Sequences of amino acids : MKHHHHHHAS VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQM
Storage : Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
IL6 Crystal Structure :

Gene Name : IL6 interleukin 6 (interferon, beta 2) [ Homo sapiens ]
Official Symbol : IL6
Synonyms : IL6; interleukin 6 (interferon, beta 2); IFN-beta-2; IL-6; BSF2; BSF-2; HGF; HSF; IFNB2; CDF; Hybridoma growth factor; Interferon beta-2; B-cell stimulatory factor 2; B cell stimulatory factor-2; B-cell differentiation factor; interleukin BSF-2; interleukin-6; CTL differentiation factor; OTTHUMP00000158544; OTTHUMP00000198486; OTTHUMP00000198490
Gene ID : 3569
mRNA Refseq : NM_000600
Protein Refseq : NP_000591
MIM : 147620
UniProt ID : P05231
Chromosome Location : 7q21
Pathway : African trypanosomiasis; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Intestinal immune network for IgA production
Function : cytokine activity; growth factor activity; nterleukin-6 receptor binding; contributes to interleukin-6 receptor binding; interleukin-6 receptor binding

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