| Cat. No. : |
ERBB2-3681H |
| Description : |
HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family. The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22). |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M Acetate buffer pH4 |
| Research topic : |
Oncology |
| Purity : |
>95% |
| Reconstitution : |
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Add DTT (5 mM ) and NaCl (0.1-0.15 M) before freezing to prevent potential aggregation. |
| Applications : |
ELISA; Western blotting |
| Quality Control Test : |
BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein |
| Sequences of amino acids : |
MTQVCTGTDM KLRLPASPET HLDMLRHLYQ GCQVVQGNLE LTYLPTNASL SFLQDIQEVQ GYVLIAHNQV RQVPLQRLRI VRGTQLFEDN YALAVLDNGD PLNNTTPVTG ASPGGLRELQ LRSLTEILKG GVLIQRNPQL CYQDTILWKD IFHKNNQLAL TLIDTNRSRA CHPCSPMCKG SRCWGESSED CQSLTRTVCA GGCARCKGPL PTDCCHEQCA AGCTGPKHSD CLACLHFNHS GICELHCPAL VTYNTDTFES MPNPEGRYTF GASCVTACPY NYLSTDVGSC TLVCPLHNQE VTAEDGTQRC EKCSKPCARG THSLPPRPAA VPVPLRMQPG PAHPVLSFLR PSWDLVSAFY SLPLAPLSPT SVPISPVSVG RGPDPDAHVA VDLSRYEG |
| Storage : |
Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C. |
