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Recombinant Human S100A4, His-tagged

Cat. No.: S100A4-3727H
Product Overview: Total 110 AA. MW: 12.84 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Description: Protein S100-A4 is a protein that in humans is encoded by the S100A4 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Source: E. coli
Species: Human
Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5.
Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications: Western blotting
Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
Sequences of amino acids: MKHHHHHHAS ACPLEKALDV MVSTFHKYSG KEGDKFKLNK SELKELLTRE LPSFLGKRTD EAAFQKLMSN LDSNRDNEVD FQEYCVFLSC IAMMCNEFFE GFPDKQPRKK
Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Gene Name: S100A4 S100 calcium binding protein A4 [ Homo sapiens ]
Official Symbol: S100A4
Synonyms: S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; OTTHUMP00000015467; OTTHUMP00000015468; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); Protein S100-A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
Gene ID: 6275
mRNA Refseq: NM_002961
Protein Refseq: NP_002952
MIM: 114210
UniProt ID: P26447
Chromosome Location: 1q12-q22
Function: RAGE receptor binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding

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