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Recombinant Human S100A8, His-tagged

Cat. No.: S100A8-3732H
Product Overview: Total 103 AA. MW: 12.08 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Description: Protein S100-A8 is a protein that in humans is encoded by the S100A8 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
Source: E. coli
Species: Human
Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 100mM NaCl, pH 7.5
Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications: Western blotting
Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
Sequences of amino acids: MKHHHHHHAS MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE
Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Gene Name: S100A8 S100 calcium binding protein A8 [ Homo sapiens ]
Official Symbol: S100A8
Synonyms: S100A8; S100 calcium binding protein A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG; protein S100-A8; MRP-8; calgranulin A; OTTHUMP00000015329; cystic fibrosis antigen; calprotectin L1L subunit; urinary stone protein band A; leukocyte L1 complex light chain; migration inhibitory factor-related protein 8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium binding protein A8 (calgranulin A)
Gene ID: 6279
mRNA Refseq: NM_002964
Protein Refseq: NP_002955
MIM: 123885
UniProt ID: P05109
Chromosome Location: 1q12-q22
Function: calcium ion binding; protein binding

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