||Recombinant human MAF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-256aa).
||MAF1 that is similar to Maf1, a Saccharomyces cerevisiae protein is a protein highly conserved in eukaryotic cells and is localized to the nucleus. Interactng with BRF2, MAF1 functions to mediate signals that specifically repress the activity of RNA polymerase III (Pol III), specifically by inhibiting the assembly of TFIIIB onto DNA. It responds to changes in the cellular environment and represses pol III transcription.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
||31.0 kDa (276aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSRGSGA EETSTMEEDR VPVICI
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.