||Recombinant human CDK2 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography (1-298aa).
||CDK2, also known as cyclin-depentent kinase 2, is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
||35.0 kDa (306aa), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRLLE HHHHHH
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Cell cycle; Pathways in cancer ; Progesterone-mediated oocyte maturation; Prostate cancer; Small cell lung cancer; p53 signaling pathway; DNA Replication; Cell Cycle, Mitotic; Cell Cycle Checkpoints