||Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process (136-247aa).
||CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
||15.5 kDa (133aa)
||> 90% by SDS - PAGE
||1.0 mg/ml (determined by UV spectroscopy at 280 nm)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Cell Cycle, Mitotic; Chromosome Maintenance; DNA Replication; Deposition of New CENPA-containing Nucleosomes at the Centromere; M Phase; Mitotic M-M/G1 phases; Mitotic Prometaphase; Nucleosome assembly