Recombinant Human CENPH, His-tagged CENPH-3644H

Recombinant Human CENPH, His-tagged

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Recombinant Human CENPH, His-tagged

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Cat. No. : CENPH-3644H
Product Overview : Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process (136-247aa).
Description : CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.
Form : Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Molecular Weight : 15.5 kDa (133aa)
Purity : > 90% by SDS - PAGE
Concentration : 1.0 mg/ml (determined by UV spectroscopy at 280 nm)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Cell Cycle, Mitotic; Chromosome Maintenance; DNA Replication; Deposition of New CENPA-containing Nucleosomes at the Centromere; M Phase; Mitotic M-M/G1 phases; Mitotic Prometaphase; Nucleosome assembly
Gene Name : CENPH centromere protein H [ Homo sapiens ]
Official Symbol : CENPH
Synonyms : CENPH; ICEN35; NNF1; PMF1; centromere protein H; centromere protein H; CENP-H; OTTHUMP00000123477; OTTHUMP00000221978; OTTHUMP00000221979; kinetochore protein CENP-H; interphase centromere complex protein 35
Gene ID : 64946
mRNA Refseq : NM_022909
Protein Refseq : NP_075060
MIM : 605607
UniProt ID : Q9H3R5
Chromosome Location : 5p15.2
Function : kinetochore binding; protein binding

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