||Recombinant mouse FGF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (16-155 aa).
||Fibroblast growth factor 1, also known as FGF1, belongs to the fibroblast growth factor family. It is a potent mitogenic agent. FGF1 has been detected in large amounts in the brain. Other cells known to express FGF1 include hepatocytes, vascular smooth muscle cells, CNS neurons, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelium cells and pituitary basophils and acidophils. The growth factor acts through activation of specific cell-surface receptors leading to intracellular tyrosine phosphorylation cascade.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
||18 kDa (161aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 90% by SDS - PAGE
||< 1.0 EU per 1 μg of protein (determined by LAL method)
||1mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.