Recombinant Human ASS1, His-tagged

• Cat.No.: ASS1-30H
• Product Overview: Recombinant Human ASS1 (Met1-Lys412) fused with a 6His tag at the N-terminus, was expressed in E. coli.

Specification

• Cat. No.: ASS1-30H
• Description: The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
• Source: E. coli
• Formulation: Supplied as a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, 50mM Imidazole, 1mM DTT, 40% Glycerol, pH 7.5
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Shipping: The product is shipped on dry ice/ice packs.
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg).
• Storage: Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• OfficialSymbol: ASS1

Gene Information

• Gene Name: ASS1 argininosuccinate synthase 1 [ Homo sapiens ]
• Synonyms: ASS1; argininosuccinate synthase 1; ASS; CTLN1; argininosuccinate synthase; OTTHUMP00000022363; OTTHUMP00000022364; citrulline-aspartate ligase; citrulline--aspartate ligase; argininosuccinate synthetase 1; EC 6.3.4.5; argininosuccinate synthetase
• Gene ID: 445
• mRNA Refseq: NM_000050
• Protein Refseq: NP_000041
• MIM: 603470
• UniProt ID: P00966
• Chromosome Location: 9q34.1
• Pathway: Alanine, aspartate and glutamate metabolism; Arginine and proline metabolism; Metabolism of amino acids and derivatives
• Function: ATP binding; argininosuccinate synthase activity; protein binding; toxin binding

Reviews

09/04/2020

Critical expertise in protein engineering, a trusted research resource.

10/01/2019

Dependable peptide synthesis, accelerates our peptide-based projects effectively.

08/10/2018

Efficient protein purification, cost-effective and dependable.

Q&As

What are the mechanisms by which ASS1 modulates cell growth and proliferation?

ASS1 regulates cell growth by controlling the availability of arginine, a key amino acid for cell proliferation.

How does ASS1 deficiency contribute to the development of urea cycle disorders and hyperammonemia?

ASS1 deficiency leads to urea cycle disorders, causing hyperammonemia and metabolic imbalances.

What is the impact of ASS1 on amino acid metabolism and overall cellular function?

ASS1 affects amino acid metabolism, playing a critical role in cellular health and function.

How does ASS1 (Argininosuccinate Synthase 1) function in the urea cycle and nitrogen metabolism?

ASS1 is essential in the urea cycle, converting nitrogen waste into urea for excretion and maintaining nitrogen balance.

How do alterations in ASS1 expression or activity affect cardiovascular health and disease?

Variations in ASS1 activity can impact cardiovascular health, influencing nitric oxide production and vascular function.

What role does ASS1 play in the synthesis of arginine and its impact on nitric oxide production?

ASS1 is involved in arginine synthesis, impacting nitric oxide levels and vascular function.

How does ASS1 influence the response to high-protein diets or dietary restrictions in metabolic health?

ASS1 influences how the body metabolizes high-protein diets, affecting metabolic responses and health.